Separation of Molar Weight-Distributed Polyethylene Glycols by Reversed-Phase Chromatography—Analysis and Modeling Based on Isocratic Analytical-Scale Investigations

The separation of polyethylene glycols (PEGs) into single homologs by reversed-phase chromatography is investigated experimentally and theoretically. used core–shell column shown to achieve the baseline PEG up molar weights at least 5000 g/mol. A detailed study performed elucidating role operating conditions, including temperature, eluent composition, degree polymerization polymer. Applying Martin’s rule yields a simple model for retention times that holds wide range conditions. In combination with relations efficiency, conditions discussed, separations are predicted analytical-scale chromatography. Finally, approach included in an efficient process based on discrete convolution, which demonstrated predict high accuracy also advanced modes arbitrary injection profiles.